รบกวนเรื่องการใช้เครื่องวัด pH ค่ะ
   พอดี คู่มือกับกล่องไม่รู้ไปอยู่หนใดแล้วค่ะ เลยต้องมารบกวน พี่ๆ น้องๆ อาๆ และท่านอาจารย์ทั้งหลายค่ะ


เครื่องวัด pHที่ผมใช้เป็นแบบดิจิตอล ให้ buffer มา 3ซอง ตัวเครื่องมี 3ปุ่ม ปุ่ม hold cal และ power
1.ต้องแคริเบททั้ง 3 ตำแหน่งเลยหรือเปล่าครับ
2.ถ้าต้องทำทั้ง3ค่า เมื่อ แคริเบทที่ค่า นึงเสร็จ แล้วจะแคริเบทที่ค่าต่อไปต้องล้างหัววัดด้วยน้ำกลั่นก่อน หรือเปล่า หรือเอาไปจุ่มที่อีกค่าได้เลย แล้วถ้าทำแบบนี้จะทำให้ค่าของตัว buffer เปลี่ยนหรือไม่ หรือก่อนแคริเบท ต้องแบ่งออกมาเพื่อทำการแคริเบทแล้วทิ้ง buffer ที่แคริเบทแล้วไปเลย
3.ลำดับขั้นตอนการกดปุ่มค่ะ งงกับมันค่ะ ไม่รู้จุ่มลงไปแล้วจะกดอะไรดี แล้วถ้าจะ แคริเบทอีกค่าจะกดปุ่มอะไรยังไง

น้อง จิว กลัวทำเครื่องพังค่ะ ยังไงรบกวนด้วยนะค่ะ
ขอบคุณทุกความช่วยเหลือค่ะ น้องจิวจนปัญญาจริงๆเลยค่ะ
โดย: จิวจนปัญญาแล้วค่ะ [8 มิ.ย. 53] ( IP A:119.31.87.19 X: )
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ความคิดเห็นที่ 1
   ค้นเจอวิํธีการใช้คร่าวๆ แต่วิํธีการใช้จะ้เหมือนกันทุกตัวหรือเปล่า กล้องก็ไม่มีจะถ่ายรูปให้ดูค่้ะ ตอนนี้

การ Calibrate เครื่อง pH meter

1. เลือกกด calibration mode แล้วหน้าจอจะปรากฏข้อความ

“measure temperature buffer”

2. ให้จุ่ม pH electrode ลงใน buffer ตัวที่ 1 (pH7) หน้าจอจะเปลี่ยนเป็น

“mean U buffer” ให้รอจนกระทั่งปรากฏข้อความ “change buffer

buffer 2”

3. เปลี่ยนใส่ buffer ตัวที่ 2 (pH 4 หรือ 10 ) โดยต้องล้าง pH

electrode ก่อนด้วยน้ำกลั่นเช็ด Electrode ด้วยกระดาษทิชชูให้สะอาด

แล้วกด calibration mode จะปรากฏข้อความ measure

temperature buffer

2 แล้วเปลี่ยนเป็น meas U buffer2 ให้รอจนกระทั่งปรากฏข้อความ pH

as** สังเกตด้วยว่าเครื่องหมาย pH as ที่อ่านได้นั้นมีค่าเท่าใด แล้ว

เปลี่ยนหน้าจอกลับสู่เริ่มต้นที่เปิดเครื่อง ซึ่งตอนนี้เครื่องพร้อมที่ จะทำ

งานได้แล้ว

4. ในการวัด pH นั้น หลังจากที่จุ่ม pH electrode ลงในสารละลาย

แล้ว หน้าจอจะปรากฏข้อความ drift รอจนคำนี้หายไป และค่าตัวเลข

เริ่มนิ่ง จึงอ่านค่า

ข้อควรระวัง :

1. pH buffer ควรเก็บในตู้เย็น เมื่อต้องการใช้ควรนำออกมาวางที่

อุณหภูมิห้องก่อนจึงจะใช้ได้

2. หากสารที่ต้องการวัดมีค่าเป็น ด่างสามารถ calibrate เครื่องโดยใช้

buffer (pH=7 และ10)

3. pH electrode ควรจุ่มในสารละลายตลอดเวลา เมื่อไม่ได้วัดให้จุ่ม

electrode ในสารละลาย 3M KCl

4. ควรระมัดระวังให้ปลาย electrode สัมผัสหรือกระแทกภาชนะเพราะ

จะทำให้แตกหักได้
โดย: จิวจนปัญญาแล้วค่ะ [8 มิ.ย. 53] ( IP A:119.31.75.191 X: )
ความคิดเห็นที่ 2
   ตอบจากที่ตั้งคำถาม ไว้นะครับ
วิธีการ คร่าวๆ น่ะครับ รายละเอียดน่าจะติดต่อ Sale หรือว่าค้นหาจาก Net ได้จากยี่ห้อที่คุณใช้

1.-ใช้ช่วงไหน Cal กับ pH ช่วงค่านั้นค่าเดียวก็ได้ เช่นใช้ช่วง pH 5-6 จะ Cal 4 หรีอว่า 7 ช่วงเดียวก็ได้ หรือว่า สองช่วง ทั้ง 4และ7 ก็ได้ครับ
2.- ต้องล้างหัวก่อนที่จะ Cal ค่าใหม่ครับ // การ Cal ทุกครั้ง ถ้ามีน้ำหรือว่าสารอื่นๆ ตกค้างที่หัว+ในตัว Buffer ทำให้ค่า Buffer เพี้ยนไปครับ รวมถึงการวัดที่อุณหภูมิที่แตกต่างก็จะทำให้ค่า pH ที่ได้ต่างกันไปครับ(อุณหภูมิจะแตกต่างในทศนิยมตำแหน่งที่สองขึ้นไป ของ ตัว pH Meter)
3.- ก่อนอื่น ต้องเปิด power
-จุ่มในน้ำกลั่น สักพัก ให้ค่านิ่ง
-เช็ดหัว
-จุ่มใน Buffer
-กด Cal
ค่าจะวิ่งไปอยู่ที่ค่า Buffer ที่จุ่ม อาจจะต้องใช้ไขควงช่วย Set ค่าให้ตรงกับ Buffer
.....
ล้างหัว
ทำซ้ำจากด้านบน แต่เปลี่ยนตัว Buffer



โชคดีครับ
โดย: dUKe>duke_ry@hotmail.com [8 มิ.ย. 53 10:03] ( IP A:202.44.7.59 X: )
ความคิดเห็นที่ 3
   ขอเพิ่มคุณ Duke หน่อยครับว่า ถ้าเป็นรุ่นที่สามารถ calibrate 2 จุดได้ ก็ควรกระทำครับ
สำหรับใช้เตรียมอาหารเพาะเลี้ยงเนื้อเยื่อซึ่ง pH ราว 5-6 นั้น ควรcalibrate ที่ pH 7 และ 4 ตามลำดับครับ
โดย: สุรวิช/agrsuw@ku.ac.th [11 มิ.ย. 53 8:39] ( IP A:158.108.52.194 X: )
ความคิดเห็นที่ 4
   ขอบคุณ คุณ dUKe และคุณ สุรวิช มากค่ะ

คอยช่วยไขข้อข้องใจให้พี่ๆ เพือนๆ น้ิองๆตลอดเลยค่ัะ

ขอบคุณมากๆนะค่ะ เพื่อความชัว น้องจิว จะลองค้นดูเว็ปไซท์ผู้ผลิตอีกทีค่ะ

ขอบคุณมากค่ะ
โดย: จิวโล่งอก [11 มิ.ย. 53 13:23] ( IP A:110.49.174.77 X: )
ความคิดเห็นที่ 5
   อยากทราบว่า หาก calibrate 3 จุดไ้ด้เลย คือ ทั้ง pH 4 7 10 ควรจะทำ 3 จุดนี่จะดีกว่า calibrate เพียง 2 จุด ใช่ไหมคะ
โดย: มะปราง [1 ก.ค. 53 18:17] ( IP A:203.130.145.99 X: )
ความคิดเห็นที่ 6
   เปลืองครับ calibrate 2 จุดดีกว่า และ ไม่เปลืองครับ
บัฟเฟอร์ต้องทิ้งทุกครั้งครับ
โดย: หลุยส์ติงต๊อง [24 ก.ค. 53 19:30] ( IP A:58.9.8.19 X: )
ความคิดเห็นที่ 7
   ใครเคยเจอแบบผมบ้างไหม นำบัฟเฟอรมาจากสองที่ (จากแหล่งที่เชื่อถือได้) แต่เมื่อลองวัดเพื่อ calibrate ปรากฎว่าค่าที่ได้ของทั้งสองที่ไม่ตรงกัน ที่ Ph 7 ตรงกันแต่ ph 4 ของอันหนึ่งตรงกัน แต่ของอีกอันหนึ่งได้ ph 4.3 แล้วอย่างนี้จะเชื่องัย........เป็นไปได้ไหมว่า.......ค่าน้ำยาบัฟเฟอรผิดมาตั้งแต่ก่อนใช้แล้ว........ขอคำแนะนำจากผู้รู้ด้วยครับ...........
โดย: เด็กเก่า 68 [2 ส.ค. 53 6:47] ( IP A:125.26.1.31 X: )
ความคิดเห็นที่ 8
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โดย: t [20 พ.ย. 54] ( IP A:125.26.121.155 X: )
ความคิดเห็นที่ 9
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โดย: nmuu [24 เม.ย. 55 11:59] ( IP A:223.206.152.41 X: )
ความคิดเห็นที่ 10
   ถ้าแช่หัววัดไว้ในน้ำกลั่นจะเป็นอะไรไหมค่ะ
โดย: kam [27 พ.ย. 56 20:43] ( IP A:110.77.225.191 X: )
ความคิดเห็นที่ 11
   
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โดย: https://www.gleisontibau.com/ [31 ต.ค. 57 3:38] ( IP 91.200.12.112 X: )
ความคิดเห็นที่ 12
   

โดย: michael kors outlet [3 พ.ย. 57 16:12] ( IP 91.207.4.22 X: )
ความคิดเห็นที่ 13
   

โดย: https://michaelkorsoutlet.nkcaa.com/ [7 พ.ย. 57] ( IP 91.207.4.22 X: )
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